Molecular Formula | C24H40O5 |
Molar Mass | 408.58 |
Density | 1.0310 (rough estimate) |
Melting Point | 200-201 °C (lit.) |
Boling Point | 449.08°C (rough estimate) |
Specific Rotation(α) | 36 º (c=0.6, 95% EtOH) |
Flash Point | 9℃ |
Water Solubility | 0.28 g/L (15 ºC) |
Solubility | 1g of cholic acid is soluble in 300ml of ethanol or acetone, 7ml of glacial acetic acid, and a small amount of it is soluble in water. |
Vapor Presure | 4.59E-16mmHg at 25°C |
Appearance | White or white-like powder |
Color | White to Brown |
Merck | 14,2203 |
BRN | 2822009 |
pKa | 4.98(at 20℃) |
Storage Condition | 2-8°C |
Sensitive | Easily absorbing moisture |
Refractive Index | 1.5800 (estimate) |
MDL | MFCD00003672 |
Physical and Chemical Properties | melting point 197-202°C specific optical rotation 36 ° (c = 0.6, 95% EtOH) water-soluble 0.28g/L (15°C) |
Use | An organic acid with a steroid structure that emulsifies fat and promotes its digestion |
Hazard Symbols | Xi - Irritant![]() |
Risk Codes | 36/37/38 - Irritating to eyes, respiratory system and skin. |
Safety Description | S24/25 - Avoid contact with skin and eyes. S36 - Wear suitable protective clothing. S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. |
UN IDs | UN1230 - class 3 - PG 2 - Methanol, solution |
WGK Germany | 2 |
RTECS | FZ9350000 |
TSCA | Yes |
HS Code | 29181990 |
Raw Materials | Sulfuric acid Ethyl Alcohol |
Reference Show more | 1. Cao Yan, Song Qingqing, Li Jun, etc. Analysis of chemical constituents of bile acids in yak bile [J]. Chinese Journal of Traditional Chinese Medicine, 44 Vol. 12,, pp. 2538-2543, MEDLINE ISTIC PKU CSCD CA BP, 2019. 2. Li Wei, Jiang Zhenzhen, Li Han, Tu Pengfei, Song Qingqing, Yu Juan, Song Yuelin. Using online pressurized solvent extraction-ultra performance liquid chromatography-ion trap-Time of flight-mass spectrometry for qualitative analysis of chemical composition and grouping [J]. Chromatography, 2021,39(05):478-487. 3. Wang Jian, Sun Yu, Chen Lili, Han Zhenzhen, Sun proficient. Evaluation of different metabolites in processed products of Polygonum multiflorum Thunb based on ~ 1H-NMR metabolomics [J]. Modern medicine and clinic, 2020,35(08):1537-1543. 4. Ling, Ziting, et al. "Fabrication egg white gel hydrolysates-stabilized oil-in-water magnification and characterization of its stability and divergence." Food Hydrocolloids 102 (2020): 105621.https://doi.org/10.1016/j.foodhyd.2019.105621 5. Wu, Wanqin, et al. "Improvement of the probiotic potential and yield of Lactobacillus rhamnosus cells using corn steep liquor." LWT 131 (2020): 109862.https://doi.org/10.1016/j.lwt.2020.109862 6. Lu, Xiaoguang, et al. "Calycosin attenuates doxorubicin-induced cardiotoxicity via autophagy regulation in zebrafish models." Biomedicine & Pharmacotherapy 137 (2021): 111375.https://doi.org/10.1016/j.biopha.2021.111375 7. Song Lin, Tian-yang Wang, Hua-rong Xu, Xin-nong Zhang Qi Wang, Ran Liu, Qing Li, Kai-shun Bi,A systemic combined nontargeted and targeted LC-MS based metabolomic strategy of plasma and liver on pathology exploration of alpha-naphthylisothiocyanate induce 8. [IF=3.935] Ziying Liu et al."Promotion of classic neutral bile acids synthesis pathway is responsible for cholesterol-lowing effect of Si-miao-yong-an decoction: Application of LC-MS/MS method to determine 6 major bile acids in rat liver and plasma."J Pharmaceut Bio 9. [IF=9.147] Ziting Ling et al."Fabrication egg white gel hydrolysates-stabilized oil-in-water emulsion and characterization of its stability and digestibility."Food Hydrocolloid. 2020 May;102:105621 10. [IF=5.396] Shiming Huang et al."A sulfated polysaccharide from Gracilaria Lemaneiformis regulates cholesterol and bile acid metabolism in high-fat diet mice."Food Funct. 2019 Jun;10(6):3224-3236 11. [IF=5.34] Kaihui Zhang et al."A UPLC-MS/MS-based metabolomics analysis of the pharmacological mechanisms of rabdosia serra against cholestasis."Phytomedicine. 2021 Oct;91:153683 12. [IF=4.952] Wanqin Wu et al."Improvement of the probiotic potential and yield of Lactobacillus rhamnosus cells using corn steep liquor."Lwt Food Sci Technol. 2020 Sep;131:109862 13. [IF=3.935] Song Lin et al."A systemic combined nontargeted and targeted LC-MS based metabolomic strategy of plasma and liver on pathology exploration of alpha-naphthylisothiocyanate induced cholestatic liver injury in mice."J Pharmaceut Biomed. 2019 Jul;171:180 14. [IF=3.935] Runjing Zhang et al."Xiaoyan lidan formula ameliorates α-naphthylisothiocyanate-induced intrahepatic cholestatic liver injury in rats as revealed by non-targeted and targeted metabolomics."J Pharmaceut Biomed. 2020 Feb;179:112966 15. [IF=1.618] Chen Mengjun et al."A Powerful HPLC-ELSD Method for Simultaneous Determination of Fecal Bile Acids in T2DM Rats Interfered by Sanhuang Xiexin Tang."J Chromatogr Sci. 2021 Sep;59(9):871-876 16. [IF=5.396] Juan Wu et al."Sargassum fusiforme polysaccharide is a potential auxiliary substance for metformin in the management of diabetes."Food Funct. 2022 Feb;: 17. [IF=6.558] Yan Cao et al."Widely quasi-quantitative analysis enables temporal bile acids-targeted metabolomics in rat after oral administration of ursodeoxycholic acid."ANALYTICA CHIMICA ACTA. 2022 Jun;1212:339885 |
and this product exists in the bile of cattle, sheep and pigs. Colorless flakes or white crystalline powder. The taste is first sweet and then bitter. The melting point of the anhydrous is 198 degrees C, and the specific optical rotation is 37. (In ethanol). pK. 6.4. Soluble in solutions of alkali metal hydroxides or carbonates.
there are ethanol crystallization method and ethyl acetate separation method. The ethyl acetate separation method is to remove deoxycholic acid by dissolving ethyl acetate, and then further purify the product with ethanol. Add 1/10 sodium hydroxide to the bile of cattle and sheep, heat to boiling, and hydrolyze for 18h. The hydrolysate was cooled, the supernatant was decanted, and the lower layer was filtered. The supernatant was combined with the filtrate, and 30% sulfuric acid was added to bring the pH to 2-3, at which time crude cholic acid was floating in the liquid layer. Take the floating material, add the same amount of water to boil for 10~20min to make granular precipitation, then repeatedly rinse with water to neutral, and dry at 50~60 ℃ to obtain crude bile acid. The crude bile acid was crushed, added with 0. 75 times 75% ethanol, heated and stirred, and refluxed until the solid matter was sufficiently dissolved, and then crystallized at 0~5 °c. The crystals were filtered by suction or centrifugal spin drying, washed with a small amount of 80% ethanol and dried to obtain crude crystals of cholic acid. Take the crude crystals of cholic acid, add 4 times the amount of 95% ethanol and 4%~ 5% activated carbon, heat, stir, reflux, and filter while hot after fully dissolved. The filtrate was concentrated to about 1/4 of the volume and crystallized at 0-5 °c.
This product can be used as emulsifier.